Biomarker-based PET imaging of diffuse intrinsic pontine glioma in mouse models

Susanne Kossatz, Brandon Carney, Melanie Schweitzer, Giuseppe Carlucci, Vesselin Z. Miloushev, Uday B. Maachani, Prajwal Rajappa, Kayvan R. Keshari, David Pisapia, Wolfgang A. Weber, Mark M. Souweidane, Thomas Reiner

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

26 Zitate (Scopus)

Abstract

Diffuse intrinsic pontine glioma (DIPG) is a childhood brainstem tumor with a universally poor prognosis. Here, we characterize a positron emission tomography (PET) probe for imaging DIPG in vivo. In human histological tissues, the probes target, PARP1, was highly expressed in DIPG compared to normal brain. PET imaging allowed for the sensitive detection of DIPG in a genetically engineered mouse model, and probe uptake correlated to histologically determined tumor infiltration. Imaging with the sister fluorescence agent revealed that uptake was confined to proliferating, PARP1-expressing cells. Comparison with other imaging technologies revealed remarkable accuracy of our biomarker approach. We subsequently demonstrated that serial imaging of DIPG in mouse models enables monitoring of tumor growth, as shown in modeling of tumor progression. Overall, this validated method for quantifying DIPG burden would serve useful in monitoring treatment response in early phase clinical trials.

OriginalspracheEnglisch
Seiten (von - bis)2112-2123
Seitenumfang12
FachzeitschriftCancer Research
Jahrgang77
Ausgabenummer8
DOIs
PublikationsstatusVeröffentlicht - 15 Apr. 2017
Extern publiziertJa

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