Association of Hydrogen Peroxide Accumulation with Expression of PR-1 during Defense of Barley Against the Powdery Mildew Fungus

R. Hückelhoven, K. H. Kogel

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

2 Zitate (Scopus)

Abstract

The role of reactive oxygen intermediates (ROI) in the resistance of crop plants against their microbial pathogens is a matter of debate since several years. We here examined tissue-specific accumulation of ROI in different barley lines expressing race-specific resistance gene (Mla12, Mlg) mediated and broad spectrum (mlo5) resistance after inoculation with the barley powdery mildew fungus (Blumeria graminis f. sp. hordei). We found a genotype-specific subcellular accumulation of both Superoxide radical anions (O2-) and hydrogen peroxide (H2O2). H2O2 accumulation is linked temporally and spatially to papilla formation or the hypersensitive cell death reaction (HR) restricting pathogen growth effectively. Additionally, mRNA accumulation of a pathogenesis-related protein 1 (Hv-PR-1b) and a lipoxygenase 2 (LOX2:Hv:1), which is known to be activated after chemical induction of resistance, were analyzed in nearisogenic barley lines after inoculation with Bgh or after treatment with H2O2, respectively. Expression of these genes is discussed in context of ROI accumulation and induced resistance.

OriginalspracheEnglisch
Seiten (von - bis)231-238
Seitenumfang8
FachzeitschriftActa Phytopathologica et Entomologica Hungarica
Jahrgang35
Ausgabenummer1
PublikationsstatusVeröffentlicht - 2000
Extern publiziertJa

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