TY - JOUR
T1 - Analysis of Urinary Eicosanoids by LC-MS/MS Reveals Alterations in the Metabolic Profile after Smoking Cessation
AU - Goettel, Michael
AU - Niessner, Reinhard
AU - Scherer, Max
AU - Scherer, Gerhard
AU - Pluym, Nikola
N1 - Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/3/19
Y1 - 2018/3/19
N2 - A preceding untargeted metabolic fingerprinting approach in our lab followed by targeted fatty acid analysis revealed alterations in arachidonic acid metabolism in samples derived from a diet-controlled smoking cessation study in which compliant subjects (N = 39) quit smoking at baseline and were followed over the course of 3 months. Consequently, urinary eicosanoids were evaluated by means of a validated LC-MS/MS method. A significant decrease was obtained for the prostaglandins PGF2α, 8-iso-PGF2α, thromboxane 2,3-d-TXB2, and leukotriene E4 upon quitting smoking. These findings indicate a partial recovery of smoking-induced alterations in the eicosanoid profile due to a reduction in oxidative stress and the inflammatory response.
AB - A preceding untargeted metabolic fingerprinting approach in our lab followed by targeted fatty acid analysis revealed alterations in arachidonic acid metabolism in samples derived from a diet-controlled smoking cessation study in which compliant subjects (N = 39) quit smoking at baseline and were followed over the course of 3 months. Consequently, urinary eicosanoids were evaluated by means of a validated LC-MS/MS method. A significant decrease was obtained for the prostaglandins PGF2α, 8-iso-PGF2α, thromboxane 2,3-d-TXB2, and leukotriene E4 upon quitting smoking. These findings indicate a partial recovery of smoking-induced alterations in the eicosanoid profile due to a reduction in oxidative stress and the inflammatory response.
UR - http://www.scopus.com/inward/record.url?scp=85044106804&partnerID=8YFLogxK
U2 - 10.1021/acs.chemrestox.7b00276
DO - 10.1021/acs.chemrestox.7b00276
M3 - Article
C2 - 29400456
AN - SCOPUS:85044106804
SN - 0893-228X
VL - 31
SP - 176
EP - 182
JO - Chemical Research in Toxicology
JF - Chemical Research in Toxicology
IS - 3
ER -