TY - JOUR
T1 - Analysis of phosphorylated terpenes by electrospray ionization liquid chromatography-tandem mass spectrometry
AU - Ney, Iris
AU - Jäger, Elke
AU - Herderich, Markus
AU - Schreier, Peter
AU - Schwab, Wilfried
PY - 1996
Y1 - 1996
N2 - Linalool, nerol, geraniol, citronellol and α-terpineol were phosphorylated and the products purified by adsorption chromatography on XAD-2. High performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis using the electrospray ionization technique (ESI) revealed the occurrence of mono-, di-, tri- and tetraphosphate esters of the respective monoterpenes. In extracts obtained by XAD-2 adsorption chromatography followed by anion exchange chromatography from green tea leaves, phosphate and diphosphate esters of linalool and α-terpineol were identified for the first time by ESI LC-MS/MS. The corresponding monoterpene alcohols were analysed by high resolution gas chromatography-mass spectrometry after hydrolysis of the extract using acid phosphatase (EC 3.1.3.2).
AB - Linalool, nerol, geraniol, citronellol and α-terpineol were phosphorylated and the products purified by adsorption chromatography on XAD-2. High performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis using the electrospray ionization technique (ESI) revealed the occurrence of mono-, di-, tri- and tetraphosphate esters of the respective monoterpenes. In extracts obtained by XAD-2 adsorption chromatography followed by anion exchange chromatography from green tea leaves, phosphate and diphosphate esters of linalool and α-terpineol were identified for the first time by ESI LC-MS/MS. The corresponding monoterpene alcohols were analysed by high resolution gas chromatography-mass spectrometry after hydrolysis of the extract using acid phosphatase (EC 3.1.3.2).
KW - Atmospheric pressure ionization
KW - Electrospray mass spectrometry
KW - Green tea leaves
KW - Liquid chromatography-tandem mass spectrometry
KW - Phosphorylated terpenes
UR - http://www.scopus.com/inward/record.url?scp=0029833653&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1099-1565(199609)7:5<233::AID-PCA306>3.0.CO;2-H
DO - 10.1002/(SICI)1099-1565(199609)7:5<233::AID-PCA306>3.0.CO;2-H
M3 - Article
AN - SCOPUS:0029833653
SN - 0958-0344
VL - 7
SP - 233
EP - 236
JO - Phytochemical Analysis
JF - Phytochemical Analysis
IS - 5
ER -