Analyses of HBV cccDNA quantification and modification

Yuchen Xia; Daniela Stadler; Chunkyu Ko; Ulrike Protzer

doi:10.1007/978-1-4939-6700-1_6

Analyses of HBV cccDNA quantification and modification

Yuchen Xia, Daniela Stadler, Chunkyu Ko, Ulrike Protzer

Lehrstuhl für Virologie

Technische Universität München

Publikation: Beitrag in Buch/Bericht/Konferenzband › Kapitel › Begutachtung

32 Zitate (Scopus)

Abstract

Covalently closed circular DNA (cccDNA) serves as the transcriptional template of hepatitis B virus (HBV) replication in the nucleus of infected cells. It ensures the persistence of HBV even if replication is blocked. Immune-mediated killing of infected hepatocytes, cell division, or cytokine induced non-cytolytic degradation of cccDNA can induce the loss of cccDNA. For studies on HBV control, the analysis of cccDNA integrity and its exact quantification is very important. Here, we describe different methods for HBV cccDNA quantification and modification.

Originalsprache	Englisch
Titel	Methods in Molecular Biology
Herausgeber (Verlag)	Humana Press Inc.
Seiten	59-72
Seitenumfang	14
DOIs	https://doi.org/10.1007/978-1-4939-6700-1_6
Publikationsstatus	Veröffentlicht - 2017

Publikationsreihe

Name	Methods in Molecular Biology
Band	1540
ISSN (Print)	1064-3745

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abstract = "Covalently closed circular DNA (cccDNA) serves as the transcriptional template of hepatitis B virus (HBV) replication in the nucleus of infected cells. It ensures the persistence of HBV even if replication is blocked. Immune-mediated killing of infected hepatocytes, cell division, or cytokine induced non-cytolytic degradation of cccDNA can induce the loss of cccDNA. For studies on HBV control, the analysis of cccDNA integrity and its exact quantification is very important. Here, we describe different methods for HBV cccDNA quantification and modification.",

keywords = "Covalently closed circular DNA, Deamination, Differential DNA denaturation PCR, Quantitative PCR, Quantitative differential DNA denaturation PCR",

author = "Yuchen Xia and Daniela Stadler and Chunkyu Ko and Ulrike Protzer",

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T1 - Analyses of HBV cccDNA quantification and modification

AU - Xia, Yuchen

AU - Stadler, Daniela

AU - Ko, Chunkyu

AU - Protzer, Ulrike

N1 - Publisher Copyright: © Springer Science+Business Media LLC 2017.

PY - 2017

Y1 - 2017

N2 - Covalently closed circular DNA (cccDNA) serves as the transcriptional template of hepatitis B virus (HBV) replication in the nucleus of infected cells. It ensures the persistence of HBV even if replication is blocked. Immune-mediated killing of infected hepatocytes, cell division, or cytokine induced non-cytolytic degradation of cccDNA can induce the loss of cccDNA. For studies on HBV control, the analysis of cccDNA integrity and its exact quantification is very important. Here, we describe different methods for HBV cccDNA quantification and modification.

AB - Covalently closed circular DNA (cccDNA) serves as the transcriptional template of hepatitis B virus (HBV) replication in the nucleus of infected cells. It ensures the persistence of HBV even if replication is blocked. Immune-mediated killing of infected hepatocytes, cell division, or cytokine induced non-cytolytic degradation of cccDNA can induce the loss of cccDNA. For studies on HBV control, the analysis of cccDNA integrity and its exact quantification is very important. Here, we describe different methods for HBV cccDNA quantification and modification.

KW - Covalently closed circular DNA

KW - Deamination

KW - Differential DNA denaturation PCR

KW - Quantitative PCR

KW - Quantitative differential DNA denaturation PCR

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AN - SCOPUS:85006459327

T3 - Methods in Molecular Biology

SP - 59

EP - 72

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Analyses of HBV cccDNA quantification and modification

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