A surface plasmon resonance biochip that operates both in the angular and wavelength interrogation modes

Leiva Casemiro Oliveira, Cleumar Da Silva Moreira, Carsten Thirstrup, Elmar Uwe Kurt Melcher, Antonio Marcus Nogueira Lima, Helmut Neff

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

40 Zitate (Scopus)

Abstract

This paper presents a surface plasmon resonance system based on a polymer prism chip. The device allows operation in both the angular and wavelength interrogation modes. The biochip design is discussed emphasizing the effect of the ambient temperature over the optical behavior. Birefringence effect, biochip polishing, and responsivity are also reported. The basic mathematical formulation for both operating modes is discussed, and morphological parameters are considered in the data analysis. Experimental sensorgrams obtained at both interrogation modes with the same polymer prism chip are presented and compared. The experimental sensorgrams obtained with assays providing reversible (phosphate buffered saline and hypochlorite solutions) and irreversible (neutravindin solution) bindings demonstrate the feasibility of the proposed design.

OriginalspracheEnglisch
Aufsatznummer6408117
Seiten (von - bis)1223-1232
Seitenumfang10
FachzeitschriftIEEE Transactions on Instrumentation and Measurement
Jahrgang62
Ausgabenummer5
DOIs
PublikationsstatusVeröffentlicht - 2013
Extern publiziertJa

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