A solid-state NMR study of changes in lipid phase induced by membrane-fusogenic LV-peptides

Prashant Agrawal, Suzanne Kiihne, Johan Hollander, Mathias Hofmann, Dieter Langosch, Huub de Groot

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

9 Zitate (Scopus)

Abstract

Membrane fusion requires restructuring of lipid bilayers mediated by fusogenic membrane proteins. Peptides that correspond to natural transmembrane sequences or that have been designed to mimic them, such as low-complexity "Leu-Val" (LV) peptide sequences, can drive membrane fusion, presumably by disturbing the lipid bilayer structure. Here, we assess how peptides of different fusogenicity affect membrane structure using solid state NMR techniques. We find that the more fusogenic variants induce an unaligned lipid phase component and a large degree of phase separation as observed in 31P 2D spectra. The data support the idea that fusogenic peptides accumulate PE in a non-bilayer phase which may be critical for the induction of fusion.

OriginalspracheEnglisch
Seiten (von - bis)202-209
Seitenumfang8
FachzeitschriftBiochimica et Biophysica Acta - Biomembranes
Jahrgang1798
Ausgabenummer2
DOIs
PublikationsstatusVeröffentlicht - Feb. 2010

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