TY - JOUR
T1 - A multi-projection non-contact Tomography setup for imaging arbitrary geometries
AU - Meyer, Heiko
AU - Garofalakis, Anikitos
AU - Zacharakis, Giannis
AU - Economou, Eleftherios N.
AU - Mamalaki, Clio
AU - Papamatheakis, Sifis
AU - Ntziachristos, Vasilis
AU - Ripoll, Jorge
PY - 2005
Y1 - 2005
N2 - Optical imaging and tomography in tissues can facilitate the quantitative study of several important chromophores and fluorophores in-vivo. Due to this fact, there has been great interest in developing imaging systems offering quantitative information on the location and concentration of chromophores and fluorescent probes. However, most imaging systems currently used in reasearch make use of fiber technology for delivery and detection, which restricts the size of the photon collecting arrays leading to insufficient spatial sampling and field of view. To enable large data sets and full 360° angular measurements, we developed a novel imaging system that enables 3D imaging of fluorescent signals in bodies of arbitrary shapes in a non-contact geometry in combination with a 3D surface reconstruction algorithm. The system is appropriate for in-vivo small animal imaging of fluorescent probes. The system consists of a rotating sample holder and a lens coupled CCD camera in combination with a fiber coupled scanning device. The accuracy of the system in obtaining the surface reconstruction was measured to be in the order of 1 μm.
AB - Optical imaging and tomography in tissues can facilitate the quantitative study of several important chromophores and fluorophores in-vivo. Due to this fact, there has been great interest in developing imaging systems offering quantitative information on the location and concentration of chromophores and fluorescent probes. However, most imaging systems currently used in reasearch make use of fiber technology for delivery and detection, which restricts the size of the photon collecting arrays leading to insufficient spatial sampling and field of view. To enable large data sets and full 360° angular measurements, we developed a novel imaging system that enables 3D imaging of fluorescent signals in bodies of arbitrary shapes in a non-contact geometry in combination with a 3D surface reconstruction algorithm. The system is appropriate for in-vivo small animal imaging of fluorescent probes. The system consists of a rotating sample holder and a lens coupled CCD camera in combination with a fiber coupled scanning device. The accuracy of the system in obtaining the surface reconstruction was measured to be in the order of 1 μm.
KW - 3D reconstruction
KW - Fluorescent probes
KW - Green fluorescent protein
KW - Optical imaging
KW - T-lymphocytes
KW - Tomography
UR - http://www.scopus.com/inward/record.url?scp=25644444383&partnerID=8YFLogxK
U2 - 10.1117/12.634623
DO - 10.1117/12.634623
M3 - Conference article
AN - SCOPUS:25644444383
SN - 0277-786X
VL - 5771
SP - 244
EP - 251
JO - Proceedings of SPIE - The International Society for Optical Engineering
JF - Proceedings of SPIE - The International Society for Optical Engineering
M1 - 50
T2 - Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI
Y2 - 21 September 2004 through 24 September 2004
ER -