A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

Julian Grünewald; Ronghao Zhou; Caleb A. Lareau; Sara P. Garcia; Sowmya Iyer; Bret R. Miller; Lukas M. Langner; Jonathan Y. Hsu; Martin J. Aryee; J. Keith Joung

doi:10.1038/s41587-020-0535-y

A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

Julian Grünewald, Ronghao Zhou, Caleb A. Lareau, Sara P. Garcia, Sowmya Iyer, Bret R. Miller, Lukas M. Langner, Jonathan Y. Hsu, Martin J. Aryee, J. Keith Joung

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

198 Zitate (Scopus)

Abstract

Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR–Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors.

Originalsprache	Englisch
Seiten (von - bis)	861-864
Seitenumfang	4
Fachzeitschrift	Nature Biotechnology
Jahrgang	38
Ausgabenummer	7
DOIs	https://doi.org/10.1038/s41587-020-0535-y
Publikationsstatus	Veröffentlicht - 1 Juli 2020
Extern publiziert	Ja

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10.1038/s41587-020-0535-y

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title = "A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing",

abstract = "Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR–Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors.",

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AU - Grünewald, Julian

AU - Zhou, Ronghao

AU - Lareau, Caleb A.

AU - Garcia, Sara P.

AU - Iyer, Sowmya

AU - Miller, Bret R.

AU - Langner, Lukas M.

AU - Hsu, Jonathan Y.

AU - Aryee, Martin J.

AU - Joung, J. Keith

PY - 2020/7/1

Y1 - 2020/7/1

N2 - Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR–Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors.

AB - Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR–Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors.

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JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 7

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A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

Abstract

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