TY - JOUR
T1 - A candidate gene for fire blight resistance in Malus × robusta 5 is coding for a CC-NBS-LRR
AU - Fahrentrapp, Johannes
AU - Broggini, Giovanni A.L.
AU - Kellerhals, Markus
AU - Peil, Andreas
AU - Richter, Klaus
AU - Zini, Elena
AU - Gessler, Cesare
PY - 2013/2
Y1 - 2013/2
N2 - Fire blight is the most important bacterial disease in apple (Malus × domestica) and pear (Pyrus communis) production. Today, the causal bacterium Erwinia amylovora is present in many apple- and pear-growing areas. We investigated the natural resistance of the wild apple Malus × robusta 5 against E. amylovora, previously mapped to linkage group 3. With a fine-mapping approach on a population of 2,133 individuals followed by phenotyping of the recombinants from the region of interest, we developed flanking markers useful for marker-assisted selection. Open reading frames were predicted on the sequence of a BAC spanning the resistance locus. One open reading frame coded for a protein belonging to the NBS-LRR family. The in silico investigation of the structure of the candidate resistance gene against fire blight of M. × robusta 5, FB_MR5, led us hypothesize the presence of a coiled-coil region followed by an NBS and an LRR-like structure with the consensus 'LxxLx[IL]xxCxxLxxL'. The function of FB_MR5 was predicted in agreement with the decoy/guard model, that FB_MR5 monitors the transcribed RIN4_MR5, a homolog of RIN4 of Arabidopsis thaliana that could interact with the previously described effector AvrRpt2EA of E. amylovora.
AB - Fire blight is the most important bacterial disease in apple (Malus × domestica) and pear (Pyrus communis) production. Today, the causal bacterium Erwinia amylovora is present in many apple- and pear-growing areas. We investigated the natural resistance of the wild apple Malus × robusta 5 against E. amylovora, previously mapped to linkage group 3. With a fine-mapping approach on a population of 2,133 individuals followed by phenotyping of the recombinants from the region of interest, we developed flanking markers useful for marker-assisted selection. Open reading frames were predicted on the sequence of a BAC spanning the resistance locus. One open reading frame coded for a protein belonging to the NBS-LRR family. The in silico investigation of the structure of the candidate resistance gene against fire blight of M. × robusta 5, FB_MR5, led us hypothesize the presence of a coiled-coil region followed by an NBS and an LRR-like structure with the consensus 'LxxLx[IL]xxCxxLxxL'. The function of FB_MR5 was predicted in agreement with the decoy/guard model, that FB_MR5 monitors the transcribed RIN4_MR5, a homolog of RIN4 of Arabidopsis thaliana that could interact with the previously described effector AvrRpt2EA of E. amylovora.
KW - Apple breeding
KW - Erwinia amylovora
KW - Fine mapping
KW - Positional cloning
KW - QTL analysis
KW - R gene
UR - http://www.scopus.com/inward/record.url?scp=84872654423&partnerID=8YFLogxK
U2 - 10.1007/s11295-012-0550-3
DO - 10.1007/s11295-012-0550-3
M3 - Article
AN - SCOPUS:84872654423
SN - 1614-2942
VL - 9
SP - 237
EP - 251
JO - Tree Genetics and Genomes
JF - Tree Genetics and Genomes
IS - 1
ER -