TY - JOUR
T1 - δ13C and δ37Cl Isotope Fractionation to Characterize Aerobic vs Anaerobic Degradation of Trichloroethylene
AU - Gafni, Almog
AU - Lihl, Christina
AU - Gelman, Faina
AU - Elsner, Martin
AU - Bernstein, Anat
N1 - Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/4/10
Y1 - 2018/4/10
N2 - Trichloroethylene (TCE) is a carcinogenic organic chemical impacting water resources worldwide. Its breakdown by reductive vs oxidative degradation involves different types of chemical bonds. Hence, if distinct isotope effects are reflected in dual element (carbon and chlorine) isotope values, such trends could help distinguishing both processes in the environment. This work explored dual element isotope trends associated with TCE oxidation by two pure bacterial cultures: Pseudomonas putida F1 and Methylosinus trichosporium OB3b, where the latter expresses either soluble methane-monooxygenase (sMMO) or particulate methane-monooxygenase (pMMO). Carbon and chlorine isotope enrichment factors of TCE (ϵ13C = -11.5, -2.4, and -4.2‰ ϵ37Cl = 0.3, -1.3, and -2.4‰, respectively) differed strongly between the strains. The dual element isotope trend for strain F1 (ϵ13C/ϵ37Cl = -38) reflected, as expected, primary carbon and negligible chlorine isotope effects, whereas unexpectedly large chlorine isotope effects became apparent in the trend obtained with strain OB3b (ϵ13C/ϵ37Cl = +1.7 for sMMO and pMMO). Therefore, although dual element isotope analysis partly reflects predicted differences in oxidative vs reductive (ϵ13C/ϵ37Cl = 3.4-5.7) degradation, the unexpected OB3b fractionation data may challenge field interpretation.
AB - Trichloroethylene (TCE) is a carcinogenic organic chemical impacting water resources worldwide. Its breakdown by reductive vs oxidative degradation involves different types of chemical bonds. Hence, if distinct isotope effects are reflected in dual element (carbon and chlorine) isotope values, such trends could help distinguishing both processes in the environment. This work explored dual element isotope trends associated with TCE oxidation by two pure bacterial cultures: Pseudomonas putida F1 and Methylosinus trichosporium OB3b, where the latter expresses either soluble methane-monooxygenase (sMMO) or particulate methane-monooxygenase (pMMO). Carbon and chlorine isotope enrichment factors of TCE (ϵ13C = -11.5, -2.4, and -4.2‰ ϵ37Cl = 0.3, -1.3, and -2.4‰, respectively) differed strongly between the strains. The dual element isotope trend for strain F1 (ϵ13C/ϵ37Cl = -38) reflected, as expected, primary carbon and negligible chlorine isotope effects, whereas unexpectedly large chlorine isotope effects became apparent in the trend obtained with strain OB3b (ϵ13C/ϵ37Cl = +1.7 for sMMO and pMMO). Therefore, although dual element isotope analysis partly reflects predicted differences in oxidative vs reductive (ϵ13C/ϵ37Cl = 3.4-5.7) degradation, the unexpected OB3b fractionation data may challenge field interpretation.
UR - http://www.scopus.com/inward/record.url?scp=85047776282&partnerID=8YFLogxK
U2 - 10.1021/acs.estlett.8b00100
DO - 10.1021/acs.estlett.8b00100
M3 - Article
AN - SCOPUS:85047776282
SN - 2328-8930
VL - 5
SP - 202
EP - 208
JO - Environmental Science and Technology Letters
JF - Environmental Science and Technology Letters
IS - 4
ER -